Cochlear Fluids Simulator V 1.6h

Released April 28, 2003

Washington University Cochlear Fluids Simulation Program
Available for download free from this site

Introduction

The simulation program allows a variety of processes in the inner ear fluids to be calculated, including drug delivery by the round window, perilymph perfusion and perilymph sampling. It is useful in planning or interpreting experiments performed on the cochlea. It takes a little time to get used to how the model works, but after this it is fairly straightforward to use. If you have any trouble or want advice on what settings to use then free to contact me and I will work with you on it.

Examples of Model Output

This animation shows the calculated spread of a substance in the endolymphatic space of the guinea pig (shown uncoiled) during iontophoretic injection into the second turn. A period of 60 mins injection is being simulated. Note that diffusion along the length of the space is relatively slow, so that significant concentration differences occur between different locations. The model allows spread of drugs and other solutes in the cochlear fluids to be calculated, taking account of the volumes and geometry of the cochlear scalae and the major biophysical processes involved (diffusion, longitudinal flow, clearance, etc)

Shown below is the "standard" simulator display (click on it for a full size image) in which the spread of solute is calculated for entry through the round window membrane and for iontophoresis into endolymph of the guinea pig cochlea

This program is intended for use by researchers in the field of cochlear fluid physiology. It allows drug and other solute movements to be simulated in the inner ear under a variety of experimental conditions, including application to the round window membrane, delivery by mini-osmotic pump, perilymphatic perfusion, pressure injection, bolus injection and iontophoresis. Simulations incorporate the changing cross-sectional areas of the scalae as a function of distance and a number of solute dispersal processes, including diffusion, volume fluid flow, and clearance. Calculations can be performed based on the cochlear dimensions of any of seven species that we have available cochlear size vs distance data, including human, guinea pig, chinchilla, bat, rat, mouse and gerbil.


  Downloading, installation and operating instructions

  Documentation of Simulator Revisions (updated Apr 2003)

  Download Simulator Program v1.6h (1.7 Mb zip file)

  Help with setting the model parameters

  More examples of simulator output

  Mini Osmotic Pump Simulation in the Guinea Pig
  Round Window Application in the Guinea Pig
  Round Window Application in the Mouse

Comments, problems or requests for assistance can be sent by e-mail tosalta@ent.wustl.edu If you need any help getting the simulator started, or if you need advice on how to set parameters, please feel free to contact me. Similarly, if you have a specific experimental condition or clinical situation you want to simulate then I would be pleased to help you set it up. Your feedback, including suggestions for program modifications, is appreciated.

Development of this Simulator program was supported by research grant DC 01368 from the National Institute on Deafness and Other Communication Disorders, National Institutes of Health.